150 × 103 cells Search Results


108 tc treated 150  (AutoMate Scientific Inc)
93
AutoMate Scientific Inc 108 tc treated 150
2. Generate cancer cells for injection
108 Tc Treated 150, supplied by AutoMate Scientific Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/108 tc treated 150/product/AutoMate Scientific Inc
Average 93 stars, based on 1 article reviews
108 tc treated 150 - by Bioz Stars, 2026-02
93/100 stars
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dmem  (Thermo Fisher)
90
Thermo Fisher dmem
2. Generate cancer cells for injection
Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
dmem - by Bioz Stars, 2026-02
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ecm gel  (Millipore)
90
Millipore ecm gel
2. Generate cancer cells for injection
Ecm Gel, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
ecm gel - by Bioz Stars, 2026-02
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doxorubicin  (Millipore)
90
Millipore doxorubicin
(A, B) mRNA expression levels of CDKN2A and CDKN1A in SK-MEL-103 (A) and MEF (B) cells untreated or treated with <t>doxorubicin</t> (Doxo) or irradiation (γ-IR). Data are represented as fold change to control (mean ± SEM; pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, unpaired, two-tailed Student's t-test compared to Control). (C, D) Representative images and quantification of senescence-associated β-Gal staining of Control, Doxo-treated or γ-IR treated senescent SK-MEL-103 (C) and MEF (D) cells. Positivity to senescence-associated β-galactosidase is measured as number of ß-Gal positive cluster per field of view, mean of the positive area or percentage of β-Gal + cells over total number of cells. Data are represented as mean ± SEM (pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, **** p <0.0001 unpaired, two-tailed Student's t-test compared to Control). Scale Bar 100 μm. (E, F) Flow cytometry analysis of SPiDER-β-Gal fluorescence intensity detected in control or doxo and γ-IR treated senescent SK-MEL-103 (E) and MEF (F) cells. Transparent histograms indicate the unstained condition per each sample (one representative experiment is shown, N=2 independent experiments).
Doxorubicin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/doxorubicin/product/Millipore
Average 90 stars, based on 1 article reviews
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150-mm tissue culture dishes corning 25,383–103  (Corning Life Sciences)
90
Corning Life Sciences 150-mm tissue culture dishes corning 25,383–103
(A, B) mRNA expression levels of CDKN2A and CDKN1A in SK-MEL-103 (A) and MEF (B) cells untreated or treated with <t>doxorubicin</t> (Doxo) or irradiation (γ-IR). Data are represented as fold change to control (mean ± SEM; pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, unpaired, two-tailed Student's t-test compared to Control). (C, D) Representative images and quantification of senescence-associated β-Gal staining of Control, Doxo-treated or γ-IR treated senescent SK-MEL-103 (C) and MEF (D) cells. Positivity to senescence-associated β-galactosidase is measured as number of ß-Gal positive cluster per field of view, mean of the positive area or percentage of β-Gal + cells over total number of cells. Data are represented as mean ± SEM (pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, **** p <0.0001 unpaired, two-tailed Student's t-test compared to Control). Scale Bar 100 μm. (E, F) Flow cytometry analysis of SPiDER-β-Gal fluorescence intensity detected in control or doxo and γ-IR treated senescent SK-MEL-103 (E) and MEF (F) cells. Transparent histograms indicate the unstained condition per each sample (one representative experiment is shown, N=2 independent experiments).
150 Mm Tissue Culture Dishes Corning 25,383–103, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
150-mm tissue culture dishes corning 25,383–103 - by Bioz Stars, 2026-02
90/100 stars
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dulbecco's modified eagle medium (dmem)  (Servicebio Inc)
90
Servicebio Inc dulbecco's modified eagle medium (dmem)
(A, B) mRNA expression levels of CDKN2A and CDKN1A in SK-MEL-103 (A) and MEF (B) cells untreated or treated with <t>doxorubicin</t> (Doxo) or irradiation (γ-IR). Data are represented as fold change to control (mean ± SEM; pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, unpaired, two-tailed Student's t-test compared to Control). (C, D) Representative images and quantification of senescence-associated β-Gal staining of Control, Doxo-treated or γ-IR treated senescent SK-MEL-103 (C) and MEF (D) cells. Positivity to senescence-associated β-galactosidase is measured as number of ß-Gal positive cluster per field of view, mean of the positive area or percentage of β-Gal + cells over total number of cells. Data are represented as mean ± SEM (pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, **** p <0.0001 unpaired, two-tailed Student's t-test compared to Control). Scale Bar 100 μm. (E, F) Flow cytometry analysis of SPiDER-β-Gal fluorescence intensity detected in control or doxo and γ-IR treated senescent SK-MEL-103 (E) and MEF (F) cells. Transparent histograms indicate the unstained condition per each sample (one representative experiment is shown, N=2 independent experiments).
Dulbecco's Modified Eagle Medium (Dmem), supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dulbecco's modified eagle medium (dmem)/product/Servicebio Inc
Average 90 stars, based on 1 article reviews
dulbecco's modified eagle medium (dmem) - by Bioz Stars, 2026-02
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antibody anti acetylated lysine  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc antibody anti acetylated lysine
(A, B) mRNA expression levels of CDKN2A and CDKN1A in SK-MEL-103 (A) and MEF (B) cells untreated or treated with <t>doxorubicin</t> (Doxo) or irradiation (γ-IR). Data are represented as fold change to control (mean ± SEM; pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, unpaired, two-tailed Student's t-test compared to Control). (C, D) Representative images and quantification of senescence-associated β-Gal staining of Control, Doxo-treated or γ-IR treated senescent SK-MEL-103 (C) and MEF (D) cells. Positivity to senescence-associated β-galactosidase is measured as number of ß-Gal positive cluster per field of view, mean of the positive area or percentage of β-Gal + cells over total number of cells. Data are represented as mean ± SEM (pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, **** p <0.0001 unpaired, two-tailed Student's t-test compared to Control). Scale Bar 100 μm. (E, F) Flow cytometry analysis of SPiDER-β-Gal fluorescence intensity detected in control or doxo and γ-IR treated senescent SK-MEL-103 (E) and MEF (F) cells. Transparent histograms indicate the unstained condition per each sample (one representative experiment is shown, N=2 independent experiments).
Antibody Anti Acetylated Lysine, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody anti acetylated lysine/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
antibody anti acetylated lysine - by Bioz Stars, 2026-02
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dmem growth medium  (Thermo Fisher)
90
Thermo Fisher dmem growth medium
(A, B) mRNA expression levels of CDKN2A and CDKN1A in SK-MEL-103 (A) and MEF (B) cells untreated or treated with <t>doxorubicin</t> (Doxo) or irradiation (γ-IR). Data are represented as fold change to control (mean ± SEM; pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, unpaired, two-tailed Student's t-test compared to Control). (C, D) Representative images and quantification of senescence-associated β-Gal staining of Control, Doxo-treated or γ-IR treated senescent SK-MEL-103 (C) and MEF (D) cells. Positivity to senescence-associated β-galactosidase is measured as number of ß-Gal positive cluster per field of view, mean of the positive area or percentage of β-Gal + cells over total number of cells. Data are represented as mean ± SEM (pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, **** p <0.0001 unpaired, two-tailed Student's t-test compared to Control). Scale Bar 100 μm. (E, F) Flow cytometry analysis of SPiDER-β-Gal fluorescence intensity detected in control or doxo and γ-IR treated senescent SK-MEL-103 (E) and MEF (F) cells. Transparent histograms indicate the unstained condition per each sample (one representative experiment is shown, N=2 independent experiments).
Dmem Growth Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dmem growth medium/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
dmem growth medium - by Bioz Stars, 2026-02
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lipofectamine rnaimax  (Thermo Fisher)
86
Thermo Fisher lipofectamine rnaimax
(A, B) mRNA expression levels of CDKN2A and CDKN1A in SK-MEL-103 (A) and MEF (B) cells untreated or treated with <t>doxorubicin</t> (Doxo) or irradiation (γ-IR). Data are represented as fold change to control (mean ± SEM; pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, unpaired, two-tailed Student's t-test compared to Control). (C, D) Representative images and quantification of senescence-associated β-Gal staining of Control, Doxo-treated or γ-IR treated senescent SK-MEL-103 (C) and MEF (D) cells. Positivity to senescence-associated β-galactosidase is measured as number of ß-Gal positive cluster per field of view, mean of the positive area or percentage of β-Gal + cells over total number of cells. Data are represented as mean ± SEM (pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, **** p <0.0001 unpaired, two-tailed Student's t-test compared to Control). Scale Bar 100 μm. (E, F) Flow cytometry analysis of SPiDER-β-Gal fluorescence intensity detected in control or doxo and γ-IR treated senescent SK-MEL-103 (E) and MEF (F) cells. Transparent histograms indicate the unstained condition per each sample (one representative experiment is shown, N=2 independent experiments).
Lipofectamine Rnaimax, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
lipofectamine rnaimax - by Bioz Stars, 2026-02
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dishes falcon 3001  (Becton Dickinson)
90
Becton Dickinson dishes falcon 3001
(A, B) mRNA expression levels of CDKN2A and CDKN1A in SK-MEL-103 (A) and MEF (B) cells untreated or treated with <t>doxorubicin</t> (Doxo) or irradiation (γ-IR). Data are represented as fold change to control (mean ± SEM; pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, unpaired, two-tailed Student's t-test compared to Control). (C, D) Representative images and quantification of senescence-associated β-Gal staining of Control, Doxo-treated or γ-IR treated senescent SK-MEL-103 (C) and MEF (D) cells. Positivity to senescence-associated β-galactosidase is measured as number of ß-Gal positive cluster per field of view, mean of the positive area or percentage of β-Gal + cells over total number of cells. Data are represented as mean ± SEM (pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, **** p <0.0001 unpaired, two-tailed Student's t-test compared to Control). Scale Bar 100 μm. (E, F) Flow cytometry analysis of SPiDER-β-Gal fluorescence intensity detected in control or doxo and γ-IR treated senescent SK-MEL-103 (E) and MEF (F) cells. Transparent histograms indicate the unstained condition per each sample (one representative experiment is shown, N=2 independent experiments).
Dishes Falcon 3001, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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low melting-point agarose  (Millipore)
90
Millipore low melting-point agarose
(A, B) mRNA expression levels of CDKN2A and CDKN1A in SK-MEL-103 (A) and MEF (B) cells untreated or treated with <t>doxorubicin</t> (Doxo) or irradiation (γ-IR). Data are represented as fold change to control (mean ± SEM; pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, unpaired, two-tailed Student's t-test compared to Control). (C, D) Representative images and quantification of senescence-associated β-Gal staining of Control, Doxo-treated or γ-IR treated senescent SK-MEL-103 (C) and MEF (D) cells. Positivity to senescence-associated β-galactosidase is measured as number of ß-Gal positive cluster per field of view, mean of the positive area or percentage of β-Gal + cells over total number of cells. Data are represented as mean ± SEM (pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, **** p <0.0001 unpaired, two-tailed Student's t-test compared to Control). Scale Bar 100 μm. (E, F) Flow cytometry analysis of SPiDER-β-Gal fluorescence intensity detected in control or doxo and γ-IR treated senescent SK-MEL-103 (E) and MEF (F) cells. Transparent histograms indicate the unstained condition per each sample (one representative experiment is shown, N=2 independent experiments).
Low Melting Point Agarose, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/low melting-point agarose/product/Millipore
Average 90 stars, based on 1 article reviews
low melting-point agarose - by Bioz Stars, 2026-02
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matrigel  (Millipore)
90
Millipore matrigel
(A, B) mRNA expression levels of CDKN2A and CDKN1A in SK-MEL-103 (A) and MEF (B) cells untreated or treated with <t>doxorubicin</t> (Doxo) or irradiation (γ-IR). Data are represented as fold change to control (mean ± SEM; pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, unpaired, two-tailed Student's t-test compared to Control). (C, D) Representative images and quantification of senescence-associated β-Gal staining of Control, Doxo-treated or γ-IR treated senescent SK-MEL-103 (C) and MEF (D) cells. Positivity to senescence-associated β-galactosidase is measured as number of ß-Gal positive cluster per field of view, mean of the positive area or percentage of β-Gal + cells over total number of cells. Data are represented as mean ± SEM (pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, **** p <0.0001 unpaired, two-tailed Student's t-test compared to Control). Scale Bar 100 μm. (E, F) Flow cytometry analysis of SPiDER-β-Gal fluorescence intensity detected in control or doxo and γ-IR treated senescent SK-MEL-103 (E) and MEF (F) cells. Transparent histograms indicate the unstained condition per each sample (one representative experiment is shown, N=2 independent experiments).
Matrigel, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
matrigel - by Bioz Stars, 2026-02
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Image Search Results


2. Generate cancer cells for injection

Journal: Journal of visualized experiments : JoVE

Article Title: A Mouse Model to Investigate the Role of Cancer-associated Fibroblasts in Tumor Growth

doi: 10.3791/61883

Figure Lengend Snippet: 2. Generate cancer cells for injection

Article Snippet: Aliquot resuspended cells onto new tissue culture plates of the appropriate size with DMEM + 10% FBS (10 mL of media is suitable for a 10 cm tissue culture plate). table ft1 table-wrap mode="anchored" t5 Name of Material/Equipment Company Catalog Number Comments/Description Centrifuge for conical tubes capable of reaching 180 × g Fisher Scientific 14-432-22 Sterle 25 ml serological pipet Celltreat 667225B Sterile 10 ml serological pipet Celltreat 667210B Sterile 5 ml serological pipet Celltreat 229005B Sterile 50 ml centrifuge tubes Genesee Scientific 28-108 TC treated 150 × 20 mm dishes Genesee Scientific 25-203 TC treated 100 × 20 mm dishes Genesee Scientific 25-202 TC treated 60 × 15 mm dishes Genesee Scientific 25-260 Cell Culture Multi Flasks Fisher Scientific 14-826-95 Dulbecco’s Modified Eagle Medium Fisher Scientific 11965-118 Sterile tissue-culture grade PBS Fisher Scientific 50-751-7476 Sterile tissue culture-grade Trypsin-EDTA Fisher Scientific 15400054 Fetal bovine serum Fisher Scientific MT35010CV Countess Cell Counting Chamber Fisher Scientific C10228 Cancer cells ATCC ATCC® CRL-11147™ This is the catalog number for a primary human melanoma cell line.

Techniques: Cell Culture, Modification, Cell Counting, Derivative Assay, Injection

(A, B) mRNA expression levels of CDKN2A and CDKN1A in SK-MEL-103 (A) and MEF (B) cells untreated or treated with doxorubicin (Doxo) or irradiation (γ-IR). Data are represented as fold change to control (mean ± SEM; pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, unpaired, two-tailed Student's t-test compared to Control). (C, D) Representative images and quantification of senescence-associated β-Gal staining of Control, Doxo-treated or γ-IR treated senescent SK-MEL-103 (C) and MEF (D) cells. Positivity to senescence-associated β-galactosidase is measured as number of ß-Gal positive cluster per field of view, mean of the positive area or percentage of β-Gal + cells over total number of cells. Data are represented as mean ± SEM (pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, **** p <0.0001 unpaired, two-tailed Student's t-test compared to Control). Scale Bar 100 μm. (E, F) Flow cytometry analysis of SPiDER-β-Gal fluorescence intensity detected in control or doxo and γ-IR treated senescent SK-MEL-103 (E) and MEF (F) cells. Transparent histograms indicate the unstained condition per each sample (one representative experiment is shown, N=2 independent experiments).

Journal: Cell Stress

Article Title: A versatile method for the identification of senolytic compounds

doi: 10.15698/cst2023.12.292

Figure Lengend Snippet: (A, B) mRNA expression levels of CDKN2A and CDKN1A in SK-MEL-103 (A) and MEF (B) cells untreated or treated with doxorubicin (Doxo) or irradiation (γ-IR). Data are represented as fold change to control (mean ± SEM; pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, unpaired, two-tailed Student's t-test compared to Control). (C, D) Representative images and quantification of senescence-associated β-Gal staining of Control, Doxo-treated or γ-IR treated senescent SK-MEL-103 (C) and MEF (D) cells. Positivity to senescence-associated β-galactosidase is measured as number of ß-Gal positive cluster per field of view, mean of the positive area or percentage of β-Gal + cells over total number of cells. Data are represented as mean ± SEM (pooled from N=3 independent experiments; * p <0.05; ** p <0.01; *** p <0.001, **** p <0.0001 unpaired, two-tailed Student's t-test compared to Control). Scale Bar 100 μm. (E, F) Flow cytometry analysis of SPiDER-β-Gal fluorescence intensity detected in control or doxo and γ-IR treated senescent SK-MEL-103 (E) and MEF (F) cells. Transparent histograms indicate the unstained condition per each sample (one representative experiment is shown, N=2 independent experiments).

Article Snippet: Senescence was induced by treatment with the DNA-damage inducing agent doxorubicin (50 nM in SK-MEL-103; 150 nM in MEFs, Sigma Aldrich) or by γ-IR (7 Gy in SK-MEL-103; 10 Gy in MEFs).

Techniques: Expressing, Irradiation, Two Tailed Test, Staining, Flow Cytometry, Fluorescence